Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Hampton Research HR2-902-10/StockOptions HEPES #10 pH 7.7, 185 ml
HR2-902-10/StockOptions HEPES #10 pH 7.7, 185 ml. Please note items from this supplier are non-returnable.
Sigma Aldrich Fine Chemicals Biosciences HEPES BioPerformance Certified, >=99.5% (titration), suitable for cell culture | 7365-45-9 | MFCD00006158 | 100G
HEPES BioPerformance Certified, >=99.5% (titration), suitable for cell culture | Purity: >=99.5% (titration) | Mol Wt: 238.3 | 7365-45-9 | MFCD00006158 | 100G
Pfanstiehl Inc Tris Base (Tromethamine) High Purity, Low Endotoxin, Low Metals, USP, EP, BP, ChP (T-153) 100G GMP, Multi-compendial CAS: 77-86-1 Injectable Excipient Grade
Pfanstiehl Tris Base, High Purity, Low Endotoxin, Low Metals, USP, EP, BP, ChP is a multi-compendial buffer component that has been specifically designed and formulated to remove impurities such as endotoxin, trace metals, and nitrosamines. Manufactured in the USA under ICH-Q7 cGMP conditions, Pfanstiehl Tris Base meets parenteral (injectable) excipient grade standards, making it ideal for pharmaceutical, biologic, and vaccine bioprocessing and formulation. Pfanstiehl Brand Tris Base is compliant with USP (United States Pharmacopoeia) EP (European Pharmacopoeia), BP (British Pharmacopeia) and ChP (Chinese Pharmacopeia) Compendial, U.S.P. , cGMP , pharmaceutical grade , multicompendial
Growcells TAE BUFFER 50X 1L EA
Sterile liquid electrophoresis buffer concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: 2424.8g TRIS Base, 570ml Acetic Acid, 186.1g EDTA Ph 8.3
Vmrd Inc 4X FA Powdered Rinse Buffer
FA Rinse Buffer, pH 9.0. Powder to make one liter of 4x rinse buffer. Used to rinse fluorescent antibody antibody substrate slides.
Growcells PBS 10X 10L EA
Sterile 10X Phosphate-Buffered Saline solution concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 80g, KCl 2g, Na2HPO4 14.4g, KH2PO4 2.4g
Alkali Scientific CellPro™ TAE, 10X (Tris acetate EDTA), 4L Cubitainer
Alkali Scientific™ CellPro™ TAE Tris Acetate EDTA is a fundamental reagent in nucleic acid electrophoresis, renowned for its exceptional buffering capacity and DNA solubilization properties. Designed to provide optimal performance and reproducibility, this solution enables precise separation and analysis of DNA fragments in agarose gel electrophoresis. Each batch undergoes rigorous sterile filtration to ensure purity and consistency, empowering researchers with confidence in their molecular biology experiments.
Alkali Scientific CellPro™ TBE Running Buffer 10X, 1L
Alkali Scientific™ CellPro™ TBE (Tris-borate EDTA) solution offers a sterile and filtered formulation of this essential buffer for molecular biology and electrophoresis applications. With its meticulous preparation, this solution ensures purity and consistency, meeting the rigorous demands of scientific research and experimentation. The sterile nature of the solution minimizes the risk of contamination, making it ideal for various molecular biology techniques, including DNA and RNA agarose gel electrophoresis. Additionally, the filtered formulation guarantees clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used for nucleic acid separation, DNA fragment analysis, or other molecular biology procedures, CellPro™ TBE solution delivers consistent performance and reproducible results, supporting the success of laboratory workflows.
Teknova 10X TBST, pH 7.4. 1000mL, Sterile (0.5M Tris, 1.5M NaCl, 0.5% Tween)
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Alkali Scientific CellPro™ Tris-HCL, 1M, Stock Solution, pH 8.8, 1L
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Teknova 10X TBS with 0.5% Tween-20. (247mM Tris, 27mM KCl, 1.37M NaCl) 1000mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Alkali Scientific CellPro™ DPBS, 10X, 1L, 12/cs
Experience superior performance and convenience with Alkali Scientific™ CellPro™ 10X DPBS, Dulbecco’s Phosphate Buffered Saline. This concentrated buffer solution offers ten times the strength of standard DPBS, providing researchers with a cost-effective and time-saving option for buffer preparation. With its precisely balanced formulation and high purity, CellPro™ 10X DPBS ensures consistent results in cell culture, molecular biology, and immunocytochemistry applications. Trust Alkali Scientific™ CellPro™ 10X DPBS for reliable performance and reproducibility in your laboratory workflows.
Teknova 0.9% Saline Solution.10mL per Tube, 25 per Pack, Sterile.
Sodium chloride is an essential ingredient in the lab. Not only does it help regulate the pH of solutions, but it also prevents growth of bacteria and other contaminants. In addition, salt is a powerful desiccant, meaning that it can help to dry out wet samples. As a result, salt is often used in a wide variety of laboratory procedures.
Alkali Scientific CellPro™ MOPS Buffer 10X, Amber Bottle, 1L
Alkali Scientific™ CellPro™ MOPS Buffer 10X is a versatile reagent commonly used in molecular biology and biochemistry applications. With its optimized formulation, this buffer provides stable pH conditions, crucial for nucleic acid electrophoresis, RNA isolation, and protein gel electrophoresis. Each batch undergoes stringent sterile filtration to ensure purity and consistency, making CellPro™ MOPS Buffer 10X a trusted choice for researchers seeking reliable results in their experiments.