Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld Carbonate-buffered saline pH 9.5 (5X), 250 mL
This buffer contains 125mM sodium carbonate and 750mM sodium chloride, pH 9.5. The buffer is important for several different cellular processes. It is one of the major buffering systems used to maintain the pH of mammalian blood and is also important in the acid-base homeostasis of living things, including humans. It tends to maintain a relatively constant plasma pH and counteract any force that would alter it.Warning:For laboratory use only.Not fit for agricultural, household, clinical use or humanconsumption. Appearance: LiquidPH Value: 9.5
Teknova 20X PBS Solution with 0.1% Tween-20. 1000 mL, Non-Sterile. 2 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Kd Medical Inc PBS BUFFER, 1X, PH 7.2, 500ML
1X PBS working solution commonly used in biological research. Used as a wash buffer in several research applications. One 500ml bottle.
PLANT CELL TECHNOLOGY 1X Dulbecco's Phosphate Buffered Saline Solution without Calcium and Magnesium, Buffer Solution, Mod. 1, Clear Liquid, Sterile, 500mL bottle.
1X DPBS Mod. 1 is a sterile phosphate-buffered saline solution for mammalian cell culture applications. Provides isotonic environment to maintain cell integrity during washing, resuspension, or media preparation. Supplied as a clear liquid in a 500 mL sterile bottle. Ready-to-use and shelf stable under recommended conditions. Suitable for research, cell culture, and tissue engineering workflows.
Teknova 1X PBS with 0.1% Tween-20. 1000mL, Sterile. 2 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Apexbio Technology LLC HyperScript RT SuperMix for qPCR (with gDNA wiper) 50 rxns
HyperScript RT SuperMix for qPCR is a reverse transcription reaction premix based on HyperScript Reverse Transcriptase a novel enzyme engineered from M-MLV (RNase H-) Reverse Transcriptase featuring significantly reduced RNase H activity and greatly enhanced thermo-stability Due to its improved heat tolerance HyperScript Reverse Transcriptase is especially suitable for reverse transcription of RNA templates with complex secondary structures HyperScript RT SuperMix for qPCR (with gDNA wiper) is specifically designed for two-step qRT-PCR assays The 5 SuperMix contains all necessary components for reverse transcription Simply adding template RNA and RNase-Free ddH2O allows rapid reaction initiation The included 4 gDNA wiper efficiently eliminates potential genomic DNA contamination from samples rapidly and thoroughly prior to reverse transcription The kit is particularly suitable for reverse transcription reactions from RNA templates of low concentration
Apexbio Technology LLC Annexin V-HF488/DAPI Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF488) can be used as a fluorescent probe to detect apoptotic cells through flow cytometry or fluorescence microscopy with staining taking only 10-20 minutes DAPI specifically binds to double-stranded DNA emitting blue fluorescence and is generally unable to permeate intact cell membranes However during late apoptosis or cell death DAPI can penetrate compromised membranes enabling nuclear staining Therefore combined staining with Annexin V-HF488 and DAPI facilitates the differentiation of cells in different apoptosis stages as well as dead cells
![Research Products International Corp PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 1 Liter](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P32200-1000.031.JPG-250.jpg)
Research Products International Corp PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 1 Liter
PBS, 10X Powder Concentrate is a highly concentrated phosphate-buffered saline solution used in biology and biochemistry research. It contains sodium chloride, sodium phosphate, and potassium phosphate, which maintain pH and osmolarity. Designed for dilution before use, it offers a convenient and cost-effective way to prepare large volumes of 1X PBS. Once diluted, it serves as an isotonic buffer for cell culture, immunohistochemistry, protein work, and molecular biology. It supports cell and tissue integrity by maintaining stable physiological conditions during washing, staining, or sample preparation.